Fig. 3.

Bioinformatic analysis of TUBB8 variants. a Summary of our TUBB8 variants. The red font represents 12 novel variants, the blue font represents the 8 novel missense amino acid change occurring at the same position as another previously reported missense change, and black font represents the previously reported variants. b Conservation analysis of mutated amino acids in five different primate species. All the variant sites are highly conserved except for Q15K and L42V. c Protein conformation predictions of variants in TUBB8. Red dashed lines represent hydrogen bonds, indicated by the black arrowhead