Fig. 2.

The regulation of neuronal injury by complement C3 depends on neuronal TRPV1. A, B Western blots and analysis of C3 protein levels in brain lysates of SE (n = 3) and Ctrl (n = 3) hippocampal tissue. C, D Representative images and statistics of TRPV1 in primary hippocampal neurons after C3 treatment for 24 h. E, F Representative images and statistics of synapsin (SYN) and microtubule-associated protein 2 (MAP2) in wild-type and TRPV1-knockout hippocampal neurons after C3 treatment for 24 h. G, H Sholl analysis images and statistics of dendritic complexity in primary hippocampal neurons under C3 treatment pretreated with capsazepine (CPZ). *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant.