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. 2021 Jul 26;37(10):1427–1440. doi: 10.1007/s12264-021-00750-4

Fig. 3.

Fig. 3

The increased C3 secretion after epilepsy is derived from astrocytes. A Quantitative RT-PCR measurement of GFAP mRNA expression in brain lysates of SE (n = 4) and Ctrl (n = 5) mice. B, C Western blots and analysis of GFAP protein levels in brain lysates of SE (n = 4) and Ctrl (n = 5) mice. D, E Representative images and statistics of GFAP and C3 intensity and colocalization in SE (n = 4) and Ctrl (n = 5) mouse brain sections (green: GFAP; red: C3). F Schematic of the experimental design for astrocytes. G, H Sholl analysis images and statistics of dendritic complexity in primary hippocampal neurons under astrocyte medium treatment and pretreated with C3aRA. I, J Western blots and analysis of GFAP protein levels in primary astrocytes under 4-AP treatment. K Quantitative RT-PCR measurement of C3 mRNA expression in primary astrocytes under 4-AP treatment. *P <0.05, **P <0.01, ***P <0.001; ns, not significant.