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. 2021 Sep 21;12:726428. doi: 10.3389/fimmu.2021.726428

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Copyright © 2021 Fribourg, Cioni, Ghiggeri, Cantarelli, Leventhal, Budge, Bin, Riella, Colucci, Vivarelli, Angeletti, Perin and Cravedi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in not switched and class switched B cells after anti-CD20 Ab therapy. (A) Doughnut pie graph of the number of class switched B cells (total = average number of class switched and not-switched cells per sample) before anti-CD20 treatment (T0) and at relapse (or at the same time point after anti-CD-20 therapy in non-relapsing patients) (T2). (B) Bar graph illustrating the percentage of class switched B cells. (C) Changes in absolute numbers of not switched and class switched B cells in relapse and non-relapsing patients at T0, T1 and T2. Comparisons between the relapsing and non-relapsing groups at the same timepoint ^#p < 0.05. Comparisons between different timepoints *p < 0.05, **p < 0.01, ***p < 0.001. Two-way ANOVA corrected for multiple comparisons. Bar plots and data points depict mean ± SEM.