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. 2021 Sep 21;15:734001. doi: 10.3389/fnins.2021.734001

FIGURE 4.

FIGURE 4

ApoE3 neurons show increased neuronal activity compared to wild-type, ApoE KO and ApoE4 neurons. (A) Representative frequency distribution graphs of wild-type, ApoE KO, ApoE3 and ApoE4 neurons (18–20 DIV) obtained using live cell Ca2+ imaging. (B–E) Quantifications of spike frequency (B), percentage of independent spikes within a neuron culture (C), percentage of active neurons, i.e., more than 2 spikes per 2 min, within a culture (D), and amplitude (E) in wild-type, ApoE KO, ApoE3 and ApoE4 neurons (Nwild–type = 14 embryos, NApoE KO = 17 embryos, NApoE3 = 14 embryos, NApoE4 = 19 embryos; nwild–type = 181 neurons, nApoE KO = 432 neurons, nApoE3 = 474 neurons, nApoE4 = 327 neurons). Data are expressed as violin plots (B,C,E) or mean ± SD (D). Statistical analyses were performed using Kruskal-Wallis tests, followed by Dunn’s tests. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.