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. 2021 Sep 21;9:714011. doi: 10.3389/fcell.2021.714011

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Copyright © 2021 Deng, Tan, Dai, Luo and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of the surface modification of DBM scaffolds with hucMSC-ECM. (A) The gross appearance of the DBM scaffold with or without surface modification. (B) Scanning electron microscopy (SEM) images of scaffolds. (C) Energy dispersive spectroscopy (EDS) of scaffolds. (D) HPLC-MS/MS high-throughput analysis for the top 10 principal soluble protein components highly deposited in the DBM-ECM. (E) Protein expression of MIF tested by Western blot. Scale bar for the top panel of (B): 100 μm, Scale bar for the down panel of (B): 50 μm; Scale bar for (C): 500 μm; *p < 0.05, ****p < 0.001.