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. 2021 Sep 21;9:714011. doi: 10.3389/fcell.2021.714011

FIGURE 3.

FIGURE 3

Macrophage depletion inhibited bone regeneration induced by hucMSC-ECM. (A) Schematic outline of surgical operation model. (B) 3D images of bone regeneration of the DBM and DBM-ECM scaffolds adjacent to the femoral shaft at 4 weeks postimplantation in SCID mice with or without macrophage depletion. (C) BMD, BV/TV, and Tb. N of the regenerated bone of the DBM and DBM-ECM scaffolds at 4 weeks postimplantation. (D) Masson’s staining of the DBM and DBM-ECM scaffolds. (E) Masson’s staining of the DBM and DBM-ECM scaffolds at 4 weeks postimplantation; HB, host bone; S, scaffold fragment; blue arrowhead, necrotic tissue; red arrowhead, new blood vessels; black arrow, granulocytes. Scale bar: 1 mm for (B); Scale bar: 20 μm for (D); Scale bar for upper images of panel (E) is 20 μm and lower images of panel (E) is 50 μm; **p < 0.01, ***p < 0.005, ****p < 0.001.