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. 2021 Sep 21;9:714011. doi: 10.3389/fcell.2021.714011

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Copyright © 2021 Deng, Tan, Dai, Luo and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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hucMSC-ECM upregulated the expression of osteoinductive cytokines in macrophages via the MIF pathway. (A) The protein and phosphorylation levels of key molecules involved in the MIF signaling pathway. (B) MIF expression of hucMSCs transfected with the lentiviral vector containing mif shRNA or no shRNA; left panel: fluorescence expression of hucMSCs transfected with the GFP lentiviral vector, right image: protein level of MIF. (C) The expression of cytokines and key molecules of the MIF signaling pathway derived from macrophages in the DBM, shCtrl and shmif groups.