TABLE 2.
Summary of technologies to investigate the TIME.
| Method | Flow cytometry | Chromogenic IHC | Multiplex IHC | Spatial transcriptomics |
|---|---|---|---|---|
| Sample type | Fresh/frozen cells | FFPE/frozen tissue | FFPE tissue | Frozen tissue |
| Number of markers | 18+ | 2+ | Up to 8 | Whole transcriptome |
| Detection system | Antibody | Enzymatic reaction | Enzymatic reaction | Barcoded primers |
| Read out | Fluorescent | Chromogenic | Fluorescent | Sequencing |
| Co-expression | Yes | No | 2+ | Not applicable |
| Soluble mediators | Yes | No | No | No |
| Cost | $ | $ | $$ | $$$ |
| Spatial information | No | Yes | Yes | Yes |
| Observer | Scientist/biologist | Scientist/pathologist | Scientist/pathologist | Bio-informatician |
| Clinical relevant | No | Yes | Yes | No |