FIGURE 1.

SALL1 and CBX4 do not colocalize in nuclear bodies. (A–F) Confocal images of U2OS cells showing expression of SALL1-YFP, SALL1ΔSUMO-YFP, or SALL1ΔSIM-YFP (green), and endogenous CBX4 (magenta in A–C) or endogenous SUMO2/3 (magenta in D–F). Nuclei were stained with DAPI. Black and white pictures show single green or magenta channels. Green arrowheads indicate SALL1 bodies, magenta arrowheads indicate Pc bodies (A–C), or SUMO bodies (D–F) and white arrowheads indicate colocalization of SALL1 and SUMO2/3 (D–F). Pictures were taken with a Leica DM IRE2 confocal microscope using a 63× objective. Scale bars indicate 5 μm.