FIGURE 5.

SALL1 stabilizes CBX4 protein. (A,C) Western blot analysis of cycloheximide (CHX) chase experiments performed in HEK 293FT cells transfected with SALL1-YFP, SALL1ΔSUMO-YFP, or GFP-β-gal. Cells were treated with 50 μg/ml of CHX in the absence (A) or presence (C) of 10 μM of the proteasome inhibitor MG132. Cells were collected at different time points (0, 4, 8, and 16 h after initiation of treatment) and endogenous CBX4 levels were analyzed by Western blot. Vinculin was used as loading control. Molecular weight markers are shown to the right in KDa. Antibodies were used as indicated to the left. (B,D) CBX4 levels were quantified after CHX treatment alone (B) or in combination with MG132 (D), normalized to Vinculin, and data from six different independent experiments were pooled together. Graphs show mean plus SEM. P-values were calculated using one-way ANOVA test. *P-value < 0.05; **P-value < 0.01.