FIGURE 7.

SALL1 expression increases the number and size of CBX4-containing Pc bodies and enhances downregulation of CBX4 targets. (A,B) Graphs represent the number of CBX4-containing Pc bodies (A) and their mean area in pixels quantified using Fiji software (B) in U2OS cells expressing SALL1-YFP, SALL1ΔSUMO-YFP, or GFP-β-gal as a negative control. (C) Graph showing the mRNA expression levels of several CBX4 target genes in HEK 293FT cells expressing SALL1-YFP, SALL1ΔSUMO-YFP, or GFP-β-gal as control. Data shown correspond to the mean plus SEM of at least five independent RT-qPCR experiments. Gene expression data were normalized to GAPDH and are shown as relative fold change over β-Gal expressing cells (magenta line). P-values were calculated using one-way ANOVA test. *P-value < 0.05; **P-value < 0.01; ***P-value < 0.001.