TABLE 1.
Plasmids used in the study.
| Name of the vector | References | Parental vectors | Cloning sites/notes |
| CAG-bioSUMO3-T2A-BirAopt-T2A-GFPpuro | Pirone et al., 2017 | – | – |
| CMV-CBX4-YFP | This work | pEYFP-N1 | EcoRI-SalI (KAN); CBX4 generated by high-fidelity PCR |
| CMV-SALL1-YFP | Pirone et al., 2017 | pEYFP-N1 | EcoRI-SalI (KAN); SALL1 generated by high-fidelity PCR |
| CMV-SALL1ΔSUMO-YFP | This work | CMV-SALL1-YFP | EcoRI-SalI; mutants introduced by overlap extension PCR (KAN); K571R; K592R; K982R; K1086R |
| CMV-SALL1ΔSIM-YFP | This work | CMV-SALL1-YFP | EcoRI-SalI; mutants introduced by overlap extension PCR (KAN); predicted SIMs mutated to AAAA; SIM71: VLIV; SIM195: VIIE; SIM254: ILLL; SIM1252: ISVI |
| CMV-SALL1-2xHA | This work | CMV-SALL1-YFP | EYFP exchanged for 2xHA using SalI-NotI (KAN) |
| CMV-SALL1826-2xHA | This work | CMV-SALL1(826)-YFP | EYFP exchanged for 2xHA using SalI-NotI (KAN) |
| CB6-HA-N | M. Way lab (CRUK, London) | CB6 | CB6 has CMV promoter and confers neo selection; contains N-terminal HA epitope and MCS (AMP) |
| CMV-EGFP-β-galactosidase | This work | pEGFP-N1 | LacZ subcloned from pIND/lacZ (Invitrogen) |
| CB6-HA-SALL1 | This work | CB6-HA-N | SALL1 from CMV-SALL1-YFP |
| CB6-HA-SALL1ΔSUMO | This work | CB6-HA-N | SALL1 from CMV-SALL1 ΔSUMO-YFP |
| CB6-HA-CBX4 | This work | CB6-HA-N | CBX4 from CMV-CBX4-YFP |
| CMV-SALL1-BirA* | This work | CMV-SALL1-YFP | Exchanged YFP for BirA*(BioID) by Sal1-Not1 |
| CMV-Pc-BirA* | This work | CMV-SALL1-BirA* | Drosophila Pc (PCR amplified) exchanged for SALL1 using EcoR1-Sal1 (KAN); Pc source: Addgene #1927 |
| CMV-CBX4-BirA* | This work | CMV-SALL1-BirA* | CBX4 (PCR amplified) exchanged for SALL1 using EcoR1-Sal1 (KAN) |
| CMV-BirAopt-2A-puro | Pirone et al., 2017 | – | – |
| CMV-bioUB-2A-BirAopt-2A-puro | Pirone et al., 2017 | – | – |
| LL-CMV-GFS-SALL1-IRES-puro | This work | LL-CMV-GFS-IRES-puro | SALL1 inserted into modified version of Lentilox3.7; expresses N-terminal GFP-FLAG-STREP tag |
| TripZ-SALL1-2xHA-puro | This work | CMV-SALL1-2xHA; TRIPZ | Inserted SALL1-2xHA amplicon into BshT1-Mlu1TRIPZ (Dharmacon) |
| pcDNA3 | Invitrogen | – | – |
| Lenti-Cas9-blast vector | Addgene #52962 | – | – |
| psPAX2 | Addgene #12260 | – | – |
| pMD2.G (VSV-G envelope) | Addgene #12259 | – | – |
| pEYFP-N1, pEYFP-C | Clontech | – | – |
KAN or AMP indicate the antibiotic resistant cassette (kanamycin or ampicillin, respectively) in the vector for bacterial transformation.