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. 2021 Sep 21;12:688493. doi: 10.3389/fimmu.2021.688493

Figure 3.

Figure 3

Smc3 haploinsufficient cells undergo proliferation burst but fail to differentiate into plasma cells. (A) Scheme depicting mature B cell differentiation leading to the plasma cell phenotype. (B) Uniform Manifold Approximation and Projection (UMAP) applied to single cell RNA-seq populations of germinal center and post germinal center populations. (C) UMAP projections of gene expression cell signatures used to classify clusters. (D) Applying previously defined gene expression signatures, Seurat clusters were manually defined as centrocyte (CC), pre-memory B cells (Pre-MBC), transitioning centroblast to centrocyte (CB → CC), transitioning centrocyte to centroblast (Recycling), memory B cells (MBC), plasmablast (PB), and plasma cells (PC). (E) UMAP showing the projections of in vivo LPS-stimulated CFSE stained B cells that divided 8 times expressing CD138 (D8 CD138+) or not (D8 CD138). (F) Violin plots showing expression levels of D8 CD138+ for PB and PC clusters. (G) Violin plots showing expression levels of D8 CD138 for PB and PC clusters. (H) Cell densities for pseudotime lineage (CB → CB to CC → CC → PB → PC, top plot) and scatter plots of cells by genotype across pseudotime lineage expressing D8 CD138+ (middle panel) or D8 CD138 (lower panel) profiles. ****p < 0.00001.