Figure 4.

Smc3 haploinsufficiency increases DNA damage in germinal center B cells. (A–D) Gating strategy used to detect phospho-γH2AX by flow cytometry (left) and quantification (right) in B cells (A), germinal centers (B), centroblasts (C), and centrocytes (D). (E) Identification and classification of chromosomal aberrations in induced GC B cells (iGC). (F) Quantification of the frequency of chromosomal breaks and centromeric fusions in iGCs of Smc3^wt/wt (n=3) and Smc3^wt/– (n=3) mice, and (G), quantification of chromosomal breaks, (H), centromeric fusions per cell, or (I) total events per cell. Experiment shown is a representative one from 3 performed. P values calculated using a binomial test for expected versus observed frequencies (F) and Wilcoxon rank test for count distribution (G-I).