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. 2021 Sep 21;9:697831. doi: 10.3389/fcell.2021.697831

FIGURE 3.

FIGURE 3

Validation of the interactions among RP11-499E18.1, PAK2, and SOX2. (A–C) There was no significant difference of SOX2 expression between normal tissues and OC tissues. (D,E) High expression of SOX2 was closely associated with poor prognosis of OC patients. (F) The band located at 60 kDa was identified as PAK2. (G) RNA pulldown assay affirmed that there was an interaction between RP11-499E18.1 and PAK2. (H,I) Domain mapping assay and catRAPID database analysis confirmed that there was a strong binding effect between the 150–200 bp region of lncRNA RP11-49E18.1 and the 126–177/245–275 amino acid region of PAK2. (J) There was an interaction between PAK2 and SOX2 in CaOV3 and SKOV3 cells. (K–M) The expression of PAK2 and SOX2 in CaOV3 and SKOV3 cells was notably higher than in IOSE80 cells. (N) The expression of PAK2 and SOX2 in OC tissues was much higher than in pericarcinomatous tissues. OC, ovarian cancer; PAK2, P21 (RAC1) activated kinase 2; SOX2, SRY-box transcription factor 2. #p < 0.05, ##p < 0.01, ###p < 0.001.