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. 2021 Sep 21;9:697831. doi: 10.3389/fcell.2021.697831

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Copyright © 2021 Yang, Peng and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RP11-499E18.1 overexpression obviously suppressed cell proliferation, migration, colony formation, and EMT transformation. The sequences of NC and si-RP11-499E18.1 were respectively transfected into CaOV3 and SKOV3 cells to silence RP11-499E18.1 expression. The plasmids pcDNA 3.1 and pcDNA-RP11-499E18.1 were respectively transfected into CaOV3 and SKOV3 cells to overexpress RP11-499E18.1. (A,B) The transfection efficiency was high, and RP11-499E18.1 was successfully silenced and overexpressed in CaOV3 and SKOV3 cells. (C,D) There was no obvious influence of RP11-499E18.1 knockdown or overexpression on the expression of PAK2. (E–H) The proliferation, colony formation, and migration of CaOV3 and SKOV3 cells were distinctly repressed by RP11-499E18.1 overexpression, while notably promoted by RP11-499E18.1 knockdown. (I) The expression of EMT-related E-cadherin was distinctly increased, while Vimentin was notably decreased by RP11-499E18.1 overexpression. RP11-499E18.1 knockdown performed the opposite effects. NC, negative control; si, small interfering; PAK2, P21 (RAC1) activated kinase 2; EMT, epithelial–mesenchymal transition. ^#p < 0.05, ^###p < 0.001.