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. 2021 Sep 21;12:707977. doi: 10.3389/fimmu.2021.707977

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Copyright © 2021 Jiang, Zhang, Liu, Ding, Liu, Tian, Wang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunization strategies and antibody responses in mice. (A) Scheme of immunization and sampling. Serum samples were collected 2 weeks after each immunization. Each immunogen plus with Freund’s adjuvant by subcutaneous (s.c.) injection for the immunizations at 0, 14, and 28 dpi. The mice with the highest antibody titers in each group were given the 3rd boost by intravenous (i.v.) injection at 42 dpi for mAbs preparation. (B) Titers of serum samples at 14, 28, and 42 dpi were detected by ELISA. Data was shown as mean ± SEM. ***p < 0.001. Graphs were made in GraphPad Prism version 8.0.2.