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. 2021 Sep 21;11:690618. doi: 10.3389/fonc.2021.690618

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Copyright © 2021 Jiang, Zhou, Shi, Feng and Lyu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SMYD3 and ITGB6 can activate the TGFβ1/Smad3 pathway and upregulate the expression of EMT-related genes in 3D-cultured ovarian cancer spheroids. (A) The changes in the phosphorylation level of Smad3 and the expression of SMYD3, ITGB6, N-cadherin, Snail, Vimentin and E-cadherin in 3D-cultured HEY and A2780 cells were evaluated by western blot analysis after SMYD3 silencing. (B) The changes in the phosphorylation level of Smad3 and the expression of ITGB6, N-cadherin, Snail, Vimentin and E-cadherin in 3D-cultured HEY and A2780 cells were evaluated by western blot analysis after ITGB6 silencing. (C) Immunofluorescence assay exhibited the expression changes of F-actin, E-cadherin and N-cadherin in both 3D-cultured HEY and A2780 cells after silencing SMYD3 and ITGB6, respectively. (D) Immunofluorescence assay exhibited the expression changes of F-actin, E-cadherin and N-cadherin in both 3D-cultured HEY and A2780 cells after rhTGFβ1 treatment. In the histogram of the western blot quantification, “*” refers to p < 0.05, “**” refers to p < 0.01, and “***” refers to p < 0.001.