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. 2021 Sep 21;11:690618. doi: 10.3389/fonc.2021.690618

Figure 6.

Figure 6

TGFβ1 can promote the expression of SMYD3 and ITGB6 via feedback. (A) Changes in the expression of SMYD3 and ITGB6 in 3D-cultured HEY and A2780 cells after treatment with 10 ng/ml recombinant human TGFβ1 protein for 24, 48 and 72 h. (B) Changes in the expression of SMYD3 and ITGB6 in 3D-cultured HEY and A2780 cells after treatment with 10 μM SB431542 for 24, 48 and 72 h. (C) Changes in the expression of SMYD3 and ITGB6 in 3D-cultured HEY and A2780 cells after treatment with or without rhTGFβ1 and SB431542. RhTGFβ1 protein (10 ng/ml) was added to the culture medium and incubated for 72 h. Then, 10 μM/ml SB431542 was added to the medium and incubated for 6 h (equal doses of PBS and DMSO were used as the negative controls for rhTGFβ1 and SB431542, respectively). (D) Changes in the protein expression of SMYD3 and ITGB6 in 3D-cultured HEY and A2780 cells after treatment with or without latent TGFβ1 and anti-ITGB6. In the histogram of the western blot quantification, “*” refers to p < 0.05 and “**” refers to p < 0.01.