FIGURE 3.

circDTL negatively regulates the expression of miR-1287-5p in NSCLC cells. (A) Cellular location of GAPDH, U6, and circDTL was assayed in NSCLC cells. (B) RNA pull-down assay was used for the detection of circDTL. (C) RNA pull-down was performed to examine different miRNAs that bind with circDTL. (D) Predictive binding sites between circDTL and miR-1287-5p (left), the interaction between miR-1287-5p and circDTL was evaluated by dual-luciferase reporter assay (right). (E) NSCLC cells were transfected with shNC or shRNAs against circDTL (sh#1, sh#2) for 24 h, levels of miR-1287-5p were measured. (F) The expression of miR-1287-5p in 84 pairs of NSCLC and adjacent normal tissues. Data were presented as mean ± SD. Experiments were performed at least three times. ^∗P < 0.05; ^∗∗P < 0.01.