FIGURE 4.

miR-1287-5p targets GPX4 to regulate both apoptosis and ferroptosis of NSCLC cells. (A) Predictive binding sites between miR-1287-5p and 3′-UTR of GPX4 (left), the interaction between miR-1287-5p and 3′-UTR of GPX4 was evaluated by dual-luciferase reporter assay (right). (B) NSCLC cells were transfected as indicated for 24 h, mRNA levels of GPX4 were measured by RT-PCR. (C) Protein levels of GPX4 were measured. (D) mRNA levels of GPX4 were measured in NSCLC and adjacent normal tissues. (E) RNA pull-down was conducted to evaluate the GPX4 mRNA that binds with miR-1287-5p. (F) NSCLC cells were transfected with shNC or shRNAs against circDTL (sh#1, sh#2), protein levels of GPX4 were measured. (G) mRNA levels of GPX4 were measured. (H) Protein levels of GPX4 were measured. (I) Cell viabilities were measured at different time points. (J) cell migration distance was calculated. (K) Several invaded cells were measured. (L) Cell death was measured. (M) the release of mitochondrial proteins was measured. (N) Levels of ROS, MDA, GSH, and Fe² + were measured. Data were presented as mean ± SD. Experiments were performed at least three times. ^∗P < 0.05; ^∗∗P < 0.01.