TABLE 3.

Kinetic properties of Tap1a-WT and Tap1a-OPT1 at hNa_V1.1 and hNa_V1.7 determined via patch-clamp electrophysiology. Kinetic data were determined using peptide concentrations equivalent to 10× IC₅₀, and k _obs and k _off are given as the mean ± SEM from 4–7 independent experiments.

	Kon observed (s−1)	Kon (nM−1s−1)	Koff (s−1)	Kd (nM)
hNaV1.1
Tap1a-WT	2.01 ± 0.1 × 10−2 †	6.53 × 10–6	6.56 ± 0.32 × 10−4	3.16 × 10–2
Tap1a-OPT1	8.75 ± 0.8 × 10−2 * , #	ndb	irreversiblea	ndb
hNaV1.7
Tap1a-WT	7.07 ± 1.2 × 10−3	6.52 × 10–6	8.46 ± 0.6 × 10–4	1.2 × 10–1
Tap1a-OPT1	8.60 ± 1.7 × 10−3	ndb	irreversiblea	ndb

K_on observed = 1/τ_on; k_on = [(1/τ_on)–k_off]/(ligand); k_{off =} 1/τ_off; K_d = k_off /k_on.

^* Statistically significant compared to Tap1a-WT K_on observed for Na_V1.1, p = 0.0121.

^† Statistically significant compared to Tap1a-WT K_on observed for Na_V1.7, p = 0.0061.

^# Statistically significant compared to Tap1a-OPT1 K_on observed for Na_V1.7, p = 0.0012.

^aK_off is less than the lowest valid measurement under the experimental conditions.

^bnd = not determined.