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. 2021 Oct 4;41(10):BSR20210486. doi: 10.1042/BSR20210486

Figure 5. USP52 stabilizes PTEN protein in NSCLC cells.

Figure 5

(A) Control or USP52-HA plasmids were transfected into H292 cells for 48 h, followed by Western blot against PTEN and HA. (B) The relative abundance of PTEN in H292 from (A) was quantified by gray scanning after USP52-HA transforming at 0, 1, 2, 4 μg. These experiments were repeated for four times. One-way ANOVA, *P<0.05, **P<0.01. NS, not significant. (C) qRT-PCR against PTEN after USP52-HA transformed at 0, 1, 2, 4 μg. (D) H292 cells were transfected with USP52-HA or empty vector (Control) for 24 h, followed by CHX chase assay at indicated time. Then, Western blot was performed to assess the expression levels of PTEN and USP52-HA. (E) Statistical analysis of (D). (F) H292 cells were transfected with USP52-HA plasmid (control) and treated with MG-132 respectively, and then Western blot was performed on PTEN, USP52 and GADPH, where - means untreated (control). (G) The relative abundance of PTEN, USP52 and GADPH in H292 from (F) was analyzed by quantitative statistical analysis, **P<0.01. NS, not significant.