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. 2021 Sep 21;12:725997. doi: 10.3389/fmicb.2021.725997

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Copyright © 2021 Grigorian, Groisillier, Thomas, Leblanc and Delage.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Screening of ZgHAD specificity toward different halogenated substrates based on colorimetric assays. Pictures were taken after 0, 5, 10 and 30 min of enzymatic reaction. Substrates are: (1) iodoacetic acid; (2) bromoacetic acid; (3) chloroacetic acid; (4) D-2-bromopropionic acid; (5) L-2-bromopropionic acid; (6) D-2-chloropropionic acid; (7) L-2-chloropropionic acid; (8) trichloroacetic acid; (9) 3-iodopropionic acid; (10) 3-bromopropionic acid; (11) 2-chlorobutyric acid; (12) iodoacetamide; (13) dibromoacetic acid; (-) no enzyme; (+) with enzyme.