Table 1.
Characterization of the specialized metabolites in Fushen using UPLC-Q/TOF-MS in ESI−.
| No. | tR (min) | Molecular formula | Adduct ion (mass accuracy, ppm) | MS/MS Fragmentation (mass accuracy, ppm <10) | Identification | References |
|---|---|---|---|---|---|---|
| 1 | 1.53 | C29H38O14 | 609.2192[M-H]−(1.5) | 347.1870 | Unknown | |
| 2 | 1.67 | C20H26O5 | 345.1689[M-H]−(−3.8) | Unknown | Zhu et al., 2018b | |
| 3 | 1.73 | C20H30O5 | 349.1995[M-H]−(−5.7) 395.2063[M-H+HCOOH]−(−1.8) |
265.1440 | Pregn-7-ene-2β,3α,15α,20(S)-tetrol | Deng et al., 2020 |
| 4 | 1.87 | C20H32O5 | 351.2140[M-H]−(−8.8) 397.2187[M-H+HCOOH]−(−9.8) |
317.1723 | Unknown | |
| 5 | 1.97 | C20H28O4 | 331.1883[M-H]−(−7.9) | Unknown | Zhu et al., 2018b | |
| 6 | 2.09 | C20H28O4 | 331.1883[M-H]−(−7.9) | Unknown | Zhu et al., 2018b | |
| 7 | 2.20 | C17H24O4 | 291.1574[M-H]−(−7.6) | Unknown | Zhu et al., 2018b | |
| 8 | 2.43 | C20H26O4 | 329.1765[M-H]−(3.6) | 251.1639 | Unknown | Zhu et al., 2018b |
| 9 | 2.52 | C20H28O4 | 331.1883[M-H]−(−7.9) | Unknown | Zhu et al., 2018b | |
| 10 | 2.58 | C26H46O9 | 501.3102[M-H]−(7.6) | Unknown | ||
| 11 | 2.65 | C20H32O4 | 335.2229[M-H]−(2.1) | Unknown | ||
| 12 | 2.75 | C20H32O5 | 351.2140[M-H]−(−8.8) 397.2187[M-H+HCOOH]−(−9.8) |
317.1723 | Unknown | |
| 13 | 2.86 | C20H28O4 | 331.1883[M-H]−(−7.9) | Unknown | Zhu et al., 2018b | |
| 14 | 2.96 | C20H26O4 | 329.1765[M-H]−(3.6) | 251.1639 | Unknown | Zhu et al., 2018b |
| 15 | 3.05 | C30H44O6 | 499.3052[M-H]−(−1.6) 545.3123[M-H+HCOOH]−(1.7) |
481.2930[M-H-H2O]−
425.2697[M-H-C2H5COOH]− 467.2764 327.2152 |
Poricoic acid E/Poricoic acid E isomer | Feng et al., 2018; Zhu et al., 2018b |
| 16 | 3.28 | C30H46O5 | 485.3242[M-H]−(−5.2) 531.3289[M-H+HCOOH]−(−6.2) |
441.2691[M-H-CO2]−
423.3255[M-H-CO2-H2O]− 471.3108 |
Poricoic acid G | Zhu et al., 2018b
Zou et al., 2019 |
| 17 | 3.35 | C31H48O7 | 531.3336[M-H]−(2.6) | 513.3320[M-H-H2O]−
485.3242[M-H-HCOOH]− |
Poricoic Acid K | Chen et al., 2019 |
| 18 | 3.45 | C31H46O6 | 513.3223[M-H]−(1.4) 531.3224[M-H+HCOOH]−(−8.4) |
497.2903[M-H-CH4]−
501.3239 331.1920 |
Poricoic acid D/F | Zou et al., 2019 |
| 19 | 3.57 | C31H48O5 | 499.3418[M-H]−(−1.0) 545.3458[M-H+HCOOH]−(−3.7) |
483.3095[M-H-CH4]− | Poricoic acid GM | Zou et al., 2019 |
| 20 | 3.64 | C30H44O6 | 499.3052[M-H]−(−1.6) | 481.2930[M-H-H2O]−
425.2697[M-H-C2H5COOH]− 453.3100 437.2287 |
Poricoic acid E/Poricoic acid E isomer | Feng et al., 2018; Zhu et al., 2018b |
| 21 | 3.78 | C31H48O5 | 499.3418[M-H]−(−1.0) 545.3505[M-H+HCOOH]−(5.0) 999.6895[2M-H]−(−3.0) |
481.3334[M-H-H2O]−
419.2980[M-H-HCOOH-CH4-H2O]− 437.3315 |
29-Hydroxydehydrotumulosic acid | Zou et al., 2019 |
| 22 | 4.03 | C31H46O6 | 513.3223[M-H]−(1.4) 559.3224[M-H+HCOOH]−(5.0) |
439.2852[M-H-C2H5COOH]−
487.3416 441.2992 |
5α,3β-Peroxy-dehydrotumulosicacid/ 5α,8α-Peroxy-dehydrotumulosic acid |
Wang, 2014; Zhu et al., 2018b Zou et al., 2019 |
| 23 | 4.17 | C31H46O6 | 513.3223[M-H]−(1.4) 559.3224[M-H+HCOOH]−(5.0) |
439.2809[M-H-C2H5COOH]−
487.3416 441.2992 |
5α,3β-Peroxy-dehydrotumulosicacid/ 5α,8α-Peroxy-dehydrotumulosic acid |
Wang, 2014; Zhu et al., 2018b; Zou et al., 2019 |
| 24 | 4.24 | C30H46O5 | 485.3287[M-H]−(4.1) | 467.3120[M-H-H2O]−
449.2364 |
16α,27-Dihydroxy-dehydrotrametenoic acid | Zhu et al., 2018b; Zou et al., 2019 |
| 25 | 4.30 | C30H48O5 | 487.3416[M-H]−(−1.4) 533.3452[M-H+HCOOH]−(−4.9) |
451.3192[M-H-2H2O]−
441.2261 367.2089 |
3α,16α,25-Trihydroxylanosta-8,24-dien-21-oic acid | Zou et al., 2019 |
| 26 | 4.37 | C31H48O5 | 499.3418[M-H]−(−1.0) 545.3458[M-H+HCOOH]−(−3.7) |
483.3095[M-H-CH4]−
481.3334[M-H-H2O]− 437.3420[M-H-CO2-H2O]− |
16α,25-Dihydroxyeburiconic acid/ 25-Hydroxy-3-epi-dehydrotumulosic acid |
Akihisa et al., 2009; Zou et al., 2019 |
| 27 | 4.49 | C31H46O5 | 497.3270[M-H]−(0.6) 543.3356[M-H]−(6.3) 995.6573[2M-H]−(−3.9) |
481.2930[M-H-CH4]−
419.2980[M-H-H2O-CO2-CH4]− 435.3310[M-H2O-CO2]− |
25-Hydroxyporicoic acid C/25-Hydroxypolyporenic acid C/ 6-Hydroxypolyporenic acid C/Poricoic acid BM/ 3-Oxo-6,16α-dihydroxy-lanosta-7,9(11),24(31)-trien-21-oic acid |
Li, 2013; Wang, 2016; Zhu et al., 2018b |
| 28 | 4.83 | C31H48O6 | 515.3367[M-H]−(−1.2) 561.3425[M-H+HCOOH]−(−0.4) 1031.6835[2M-H]−(1.1) |
497.3270[M-H-H2O]−
499.3418 423.2843 |
25-Hydroxyporicoic acid H | Zhu et al., 2018b |
| 29 | 4.99 | C31H48O5 | 499.3418[M-H]−(−1.0) 545.3458, [M-H+HCOOH]−(−3.7) |
481.3334[M-H-H2O]−
437.3420[M-H-CO2-H2O]− |
15α-Hydroxydehydrotumulosic acid | Zou et al., 2019 |
| 30 | 5.08 | C32H50O5 | 513.3548[M-H]−(−6.2) 559.3611[M-H+HCOOH]−(−4.3) |
471.3474 439.2809 |
3-O-Acetyl-16α-hydroxytrametenolic acid isomer | |
| 31 | 5.26 | C31H46O5 | 497.3270[M-H]−(0.6) 543.3356[M-H]−(6.3) 995.6573[2M-H]−(−3.9) |
481.2930[M-H-CH4]−
419.2980[M-H-H2O-CO2-CH4]− 483.3155 |
25-Hydroxyporicoic acid C/25-Hydroxypolyporenic acid C/ 6-Hydroxypolyporenic acid C/Poricoic acid BM/ 3-Oxo-6,16α-dihydroxy-lanosta-7,9(11),24(31)-trien-21-oic acid |
Li, 2013; Wang, 2016; Zhu et al., 2018b |
| 32 | 5.43 | C31H48O5 | 499.3418[M-H]−(−1.0) | 481.3334[M-H-H2O]− | Poricoic acid H | Zou et al., 2019 |
| 33 | 5.62 | C31H46O5 | 497.3270[M-H]−(0.6) 543.3356[M-H]−(6.3) 995.6573[2M-H]−(−3.9) |
483.3155 469.3313 467.3162 |
25-Hydroxyporicoic acid C/25-Hydroxypolyporenic acid C/ 6-Hydroxypolyporenic acid C/Poricoic acid BM/ 3-Oxo-6,16α-dihydroxy-lanosta-7,9(11),24(31)-trien-21-oic acid |
Li, 2013; Wang, 2016; Zhu et al., 2018b |
| 34 | 5.82 | C33H50O5 | 525.3594[M-H]−(2.7) 571.3607[M-H+HCOOH]−(−4.9) |
465.3363[M-CO2-CH4]−
471.3463 |
16α-Acetytoxyeburiconic | Zou et al., 2019 |
| 35 | 5.97 | C30H44O4 | 467.3162[M-H]−(0.2) 513.3177[M-H+HCOOH]−(−7.6) |
407.2950[M-H-CO2-CH4]−
393.2802[M-H-C2H5COOH]− |
16-Deoxyporicoic acid B/ 25-Methoxyporicoic acid A |
Wang, 2016; Zou et al., 2019 |
| 36 | 6.11 | C32H48O6 | 527.3361[M-H]−(−2.3) 573.3436 [M-H+HCOOH]−(1.6) |
481.3065 419.1387 |
Poricoic acid DM | Zhu et al., 2018b |
| 37 | 6.28 | C31H46O5 | 497.3316[M-H]−(9.9) | 467.3118[H-H-HCOOH]−
483.3115 |
25-Hydroxyporicoic acid C/25-Hydroxypolyporenic acid C/ 6-Hydroxypolyporenic acid C/Poricoic acid BM/ 3-Oxo-6,16α-dihydroxy-lanosta-7,9(11),24(31)-trien-21-oic acid |
Li, 2013; Wang, 2016; Zhu et al., 2018b |
| 38 | 6.41 | C31H50O4 | 485.3603[M-H]−(−5.8) 531.3666[M-H+HCOOH]−(−3.8) |
469.3623 427.2726 |
3β-Hydroxy-25-methoxy-24-methylene-27-Norlanost-8-en-21-oic acid/3-Oxo-6,16α-dihydroxy-lanosta-8,24-dien-21-oic acid | Zhu et al., 2018b
Wang, 2014 |
| 39* | 6.57 | C30H46O4 | 469.3313[M-H]−(−1.1) 515.3367[M-H+HCOOH]−(−1.2) 939.6746[2M-H]−(3.4) |
423.3264[M-H-HCOOH]−
409.3091[M-H-CO2-CH4]− 407.2993[M-H-HCOOH-CH4]− 375.2686[M-H-H2O-CO2-2CH4]− 311.1979[M-H-C8H14O2-CH4]− |
16α-Hydroxydehydrotrametenolic acid | Wang, 2016 |
| 40 | 6.81 | C31H48O4 | 483.3470[M-H]−(−0.8) 529.3495[M-H+HCOOH]−(−6.4) |
439.2552 437.2928 |
3-epi-Dehydrotumulosic acid | Wang, 2016 |
| 41* | 6.96 | C30H48O4 | 471.3463[M-H]−(−1.1) 517.3549[M-H+HCOOH]−(3.9) 943.7065[2M-H]− (4.0) |
453.3361[M-H-H2O]−
427.3487[M-H-CO2]− 409.3091[M-H-HCOOH-CH4]− |
16α-Hydroxytrametenolic acid | Zhu et al., 2018b |
| 42 | 7.16 | C33H50O6 | 541.3530[M-H]−(0.2) 587.3599[M-H+HCOOH]−(2.6) |
481.3155[M-H-CH3COOH]−
465.3010[M-H-CH3COOH-CH4]− 461.3056[M-H-HCOOH-CH4-H2O]− |
6α-Hydroxydehydropachymic acid | Zhu et al., 2018b |
| 43 | 7.23 | C31H46O4 | 481.3334[M-H]−(3.3) 527.3361[M-H+HCOOH]−(−2.3) |
407.2930[M-H-C2H5COOH]− | Poricoic acid C | Zhu et al., 2018b |
| 44 | 7.40 | C33H48O6 | 539.3406[M-H]−(6.1) | 521.2177 | 16α-Acetoxy-26,27-dimethoxyl-lanosta-8,24(31)-dien-21-oic acid | Zhu et al., 2018b |
| 45* | 7.56 | C30H44O5 | 483.3110[M-H]−(0.0) 529.3212[M-H+HCOOH]−(8.9) 967.6332[2M-H]−(4.0) |
409.2718[M-H-C2H5COOH]− | Poricoic acid B | Zhu et al., 2018b |
| 46 | 7.77 | C31H46O5 | 497.3315[M-H]−(9.7) | 483.3110 467.3029 |
25-Hydroxyporicoic acid C/25-Hydroxypolyporenic acid C/ 6-Hydroxypolyporenic acid C/Poricoic acid BM/ 3-Oxo-6,16α-dihydroxy-lanosta-7,9(11),24(31)-trien-21-oic acid |
Li, 2013; Wang, 2016; Zhu et al., 2018b |
| 47* | 7.89 | C31H48O4 | 483.3470[M-H]−(−0.8) 529.3542[M-H+HCOOH]−(2.5) 967.7032[2M-H]−(0.5) |
439.3538[M-H-CO2]−
437.3400[M-H-HCOOH]− 421.3119[M-H-HCOOH-CH4]− 389.2847[M-H-H2O-CO2-2CH4]− 311.2015[M-H-C9H16O2-CH4]− |
Dehydrotumulosic acid | Zhu et al., 2018b |
| 48 | 8.20 | C32H50O6 | 529.3542[M-H]−(2.5) | 483.3470[M-H-HCOOH]−
469.3313[M-H-CO2-CH4]− |
3β-Acetyloxy-16α,26-dihydroxy-Lanosta-8,24-dien-21-oic acid | Zhu et al., 2018b |
| 49 | 8.47 | C31H50O4 | 485.3648[M-H]−(3.5) 531.3666[M-H+HCOOH]−(−3.8) 971.7372[2M-H]−(3.3) |
469.3313[M-H-CH4]−
423.3264[M-H-HCOOH-CH4]− 391.3001[M-H- H2O-CO2-2CH4]− |
Tumulosic acid | Wang, 2016; Zhu et al., 2018b |
| 50 | 8.84 | C31H46O5 | 497.3270[M-H]−(0.6) | 479.3151[M-H-H2O]− | 25-Hydroxyporicoic acid C/25-Hydroxypolyporenic acid C/ 6-Hydroxypolyporenic acid C/Poricoic acid BM/ 3-Oxo-6,16a-dihydroxy-lanosta- 7,9(11),24(31)-trien-21-oic acid | Li, 2013
Wang, 2016 Zhu et al., 2018b |
| 51* | 9.00 | C31H46O5 | 497.3270[M-H]−(0.6) 543.3308[M-H+HCOOH]−(−2.6) 995.6658[2M-H]−(4.6) |
479.3151[M-H-H2O]−
423.2885[M-H-C2H5COOH]− 379.2993[M-H-C2H5COOH-CO2]− 363.2688[M-H-C2H5COOH-CO2-CH4]− |
Poricoic acid A | Zhu et al., 2018b |
| 52 | 9.20 | C30H46O5 | 485.3242[M-H]−(−5.2) 531.3336[M-H+HCOOH]−(2.6) |
467.3162[M-H2O]−
441.3336[M-H-CO2]− 423.2927[M-H-HCOOH-CH4]− |
Poriacosone A/Poriacosone B | Zheng and Yang, 2008; Zou et al., 2019 |
| 53 | 9.43 | C30H46O4 | 469.3313[M-H]−(−1.1) 515.3367[M-H+HCOOH]−(−1.2) |
451.3209[M-H-H2O]−
423.3011 405.2698 375.2607 |
15α-Hydroxy-3-oxolanosta-8,24-dien-21-oic acid | Zhu et al., 2018b; Zou et al., 2019 |
| 54 | 9.77 | C33H52O6 | 543.3690[M-H]−(0.7) 589.3753[M-H+HCOOH]−(2.2) |
483.3425[M-H-CO2-CH4]−
481.3289[M-H-HCOOH-CH4]− 467.3516 437.3229 |
25-Hydroxypachymic acid | Zhu et al., 2018b |
| 55 | 9.99 | C31H48O4 | 483.3515[M-H]−(8.5) 529.3542[M-H+HCOOH]−(2.5) |
439.3452, 437.3015 |
Dehydrotumulosic acid isomer | |
| 56* | 10.23 | C31H46O4 | 481.3334[M-H]−(3.3) 527.3361[M-H+HCOOH]−(−2.3) |
437.3400[M-H-CO2]−
435.3265[M-H-HCOOH]− 421.3119[M-H-CO2-CH4]− 405.2780[M-H-CO2-2CH4]− 403.2988[M-H-H2O-CO2-CH4]− |
Polyporenic acid C | Zhu et al., 2018b |
| 57 | 10.48 | C31H46O4 | 481.3289[M-H]−(−6.0) | 421.3077[M-H-CO2-CH4]−
469.3313 325.1749 |
Polyporenic acid C isomer | |
| 58 | 10.62 | C33H50O6 | 541.3530[M-H]−(0.2) | 495.3474[M-H-HCOOH]−
481.3334[M-H-CH3COOH]− 293.2087 |
29-Hydroxydehydropachymic acid | Zou et al., 2019 |
| 59 | 10.75 | C31H48O4 | 483.3470[M-H]−(0.8) 529.3495[M-H+HCOOH]−(6.4) |
439.3581[M-H-CO2]−
437.3400[M-H-HCOOH]− 423.3222[M-H-CO2-CH4]− 421.3077[M-H-HCOOH-CH4]− |
15α-Hydroxyeburiconic acid | Zou et al., 2019 |
| 60 | 10.94 | C31H48O5 | 499.3418[M-H]−(−1.0) 545.3458[M-H+HCOOH]−(−3.7) |
481.3334[M-H-H2O]−
485.3242 |
16α,29-Dihydroxyeburiconic acid | Zou et al., 2019 |
| 61 | 11.21 | C31H48O4 | 483.3470[M-H]−(0.8) 529.3542[M-H+HCOOH]−(2.5) |
439.3581[M-H-CO2]−
437.3400[M-H-HCOOH]− 423.3264[M-H-CO2-CH4]− 407.2952[M-H-CO2-2CH4]− 389.2806[M-H-H2O-CO2-2CH4]− |
16α-Hydroxyeburiconic acid | Zou et al., 2019 |
| 62 | 11.50 | C33H52O6 | 543.3642[M-H]−(−8.1) | 483.3425[M-H-CO2-CH4]− | 25-Hydroxypachymic acid isomer | Zhu et al., 2018b |
| 63 | 11.67 | C33H52O6 | 543.3642[M-H]−(−8.1) | 483.3470[M-H-CO2-CH4]− | 25-Hydroxypachymic acid isomer | Zhu et al., 2018b |
| 64 | 11.89 | C31H48O4 | 483.3425[M-H]−(−10.1) 529.3495[M-H+HCOOH]−(6.4) |
421.2951 407.2498 |
Dehydrotumulosic acid isomer | Zou et al., 2019 |
| 65 | 12.2 | C31H50O4 | 485.3648[M-H]−(3.5) | 423.3264[M-H-HCOOH-CH4]−
295.2237 |
Hispindic acid B | Zou et al., 2019 |
| 66 | 12.39 | C32H48O5 | 511.3444[M-H]−(4.1) 557.3446[M-H+HCOOH]−(−5.7) |
451.3209[M-H-CH3COOH]−
467.3162 465.3363 |
3β-Acetoxy-16α,26-dihydroxy-lanosta-8,24-dien-21-oic acid/Poricoic acid AM | Wang, 2016; Zhu et al., 2018b; Zou et al., 2019 |
| 67 | 12.59 | C32H46O5 | 509.3286[M-H]−(3.7) 555.3269[M-H+HCOOH]−(7.7) |
449.3056[M-H-CO2-CH4]− | Unknown | |
| 68 | 12.88 | C20H28O2 | 299.1999[M-H]−(−4.0) | Unknown | ||
| 69 | 13.19 | C26H56O11 | 543.3738[M-H]−(−1.1) | Unknown | ||
| 70 | 13.41 | C32H50O5 | 513.3548[M-H]−(−6.2) 559.3611[M-H+HCOOH]−(−4.3) |
469.3224 325.1823 |
3-O-Acetyl-16α-hydroxytrametenolic acid isomer | |
| 71 | 13.48 | C35H54O6 | 569.3862[M-H]−(3.5) | 16-O-Acetylpachymic acid/ 3β,15α-Bis(acetyloxy)-24-dien-21-oic acid |
Wang, 2016; Zou et al., 2019 |
|
| 72 | 13.58 | C32H48O5 | 511.3397[M-H]−(−5.1) 557.3446[M-H+HCOOH]−(−5.7) |
451.3078 353.2073 293.1737 |
3β-Acetoxy-16α,26-dihydroxy-lanosta-8,24-dien-21-oic acid /Poricoic acid AM |
Wang, 2016; Zhu et al., 2018b; Zou et al., 2019 |
| 73 | 13.81 | C32H48O5 | 511.3397[M-H]−(−5.1) 557.3446[M-H+HCOOH]−(−5.7) |
451.3078 353.2073 293.1737 |
3β-Acetoxy-16α,26-dihydroxy-lanosta-8,24-dien-21-oic acid/ Poricoic acid AM |
Wang, 2016; Zhu et al., 2018b; Zou et al., 2019 |
| 74 | 14.03 | C33H52O5 | 527.3737[M-H]−(0.2) | 511.3351 339.1989 |
Pachymic acid isomer | |
| 75* | 14.24 | C32H48O5 | 511.3397[M-H]−(−5.1) 557.3495[M-H+HCOOH]−(3.1) |
467.3516[M-H-CO2]−
465.3363[M-H-HCOOH]− 451.3209[M-H-CH3COOH]− 355.2295[M-H-C9H16O2]− |
3-O-Acetyl-16α-hydroxydehydrotrametenolic acid | Zhu et al., 2018b; Zou et al., 2019 |
| 76 | 14.43 | C33H50O5 | 525.3594[M-H]−(2.7) 571.3607[M-H+HCOOH]−(−4.9) |
463.3166[M-H-HCOOH-CH4]−
449.3058[M-H-CH3COOH-CH4]− |
3-epi-Dehydropachymic acid | Zou et al., 2019
Zhu et al., 2018b |
| 77 | 14.60 | C33H48O5 | 523.3444[M-H]−(4.0) 569.3471[M-H+HCOOH]−(−1.2) |
463.3210[M-H-CH3COOH]− | 16α-Acetoxypolyporenic acid C | Wang, 2016 |
| 78 | 14.99 | C32H50O5 | 513.3594[M-H]−(2.7) | 497.3179 | 3-O-Acetyl-16α-hydroxytrametenolic acid isomer | |
| 79 | 15.12 | C34H42O2 | 481.3199[M-H]−(2.5) | Unknown | ||
| 80* | 15.41 | C32H50O5 | 513.3594[M-H]−(2.7) 559.3660[M-H+HCOOH]−(4.5) |
453.3361[M-H-CH3COOH]−
451.3209[M-H-HCOOH-CH4]− |
3-O-Acetyl-16α-hydroxytrametenolic acid | Wang, 2014; Zhu et al., 2018b |
| 81 | 15.87 | C32H50O5 | 513.3548[M-H]−(−6.2) 559.3660[M-H-HCOOH]−(4.5) |
3-O-Acetyl-16α-hydroxytrametenolic acid isomer | ||
| 82 | 16.11 | C32H50O5 | 513.3548[M-H]−(−6.2) 559.3660[M-H-HCOOH]−(4.5) |
3-O-Acetyl-16α-hydroxytrametenolic acid isomer | ||
| 83* | 16.33 | C33H50O5 | 525.3594[M-H]−(2.7) 571.3657[M-H-HCOOH]−(3.9) |
481.3649[M-H-CO2]−
471.3509[M-H-HCOOH]− 465.3363[M-H-CH3COOH]− 463.3166[M-HCOOH-CH4]− |
Dehydropachymic acid | Zhu et al., 2018b |
| 84 | 16.82 | C31H48O4 | 483.3425[M-H]−(−10.1) 529.3495[M-H+HCOOH]−(6.4) |
465.3010 309.0355 |
Dehydrotumulosic acid isomer | Zou et al., 2019 |
| 85 | 17.09 | C33H52O5 | 527.3737[M-H]−(0.2) 573.3779[M-H+HCOOH]−(−2.1) |
467.3604[M-H-CH3COOH]−
449.3448 |
3-eip-Pachymic acid | Wang, 2016 |
| 86* | 17.56 | C33H52O5 | 527.3737[M-H]−(0.2) 573.3779[M-H+HCOOH]−(−2.1) 1055.7560[2M-H]−(0.9) |
481.3649[M-H-HCOOH]−
467.3516[M-CH3COOH]− 465.3363[M-H-HCOOH-CH4]− 339.1951[M-H-C9H16O2-2CH4]− |
Pachymic acid | Zhu et al., 2018b |
| 87 | 18.12 | C30H44O4 | 467.3162[M-H]−(0.2) 935.6389[2M-H]−−1.3 513.3177[M-H+HCOOH]−(−7.6) |
393.2802[M-H-C2H5COOH]−
449.3362[M-] |
16α-Hydroxydehydrotrametenonic | Zou et al., 2019 |
| 88 | 18.37 | C29H46O5 | 473.3311[M-H]−(9.3) | Unknown | ||
| 89 | 18.90 | C34H50O6 | 553.3514[M-H] −(−2.7) | 425.2909 367.3501 |
3,15-O-Diacetyl-dehydrotrametenolic Acid | Chen et al., 2019 |
| 90 | 19.10 | C30H44O3 | 451.3209[M-H] −(−0.7) | 433.1260 | 16α-Hydroxy-lanosta-7,9(11),24-trien-21-oic acid | Wang, 2016 |
| 91 | 19.7 | C35H50O | 485.3746[M-H] −(−7.6) | Unknown | Wang, 2014 | |
| 92 | 19.88 | C25H42O9 | 485.2791[M-H]−(8.2) | 453.3318 | Unknown | Zhu et al., 2018b |
| 93 | 19.96 | C30H46O3 | 453.3361[M-H] −(−1.8) 499.3418[M-H+HCOOH] −(−1.0) | 435.3351 409.2429 |
3β-Hydroxylanosta-7,9(11),24-trien-21-oic acid | Zhu et al., 2018b |
| 94 | 20.16 | C32H52O4 | 499.3783[M-H] −(−0.8) 545.3888[M-H+HCOOH]−(8.4) |
Unknown | ||
| 95 | 20.45 | C23H32O2 | 339.2328[M-H]−(1.2) | Unknown | ||
| 96 | 20.72 | C31H46O4 | 481.3334[M-H]−(3.3) 527.3361[M-H+HCOOH] −(−2.3) |
437.3400[M-CO2]−
421.3119[M-CO2-CH4]− |
Polyporenic acid C isomer | |
| 97 | 20.94 | C38H52O5 | 587.3748[M-H]−(2.0) | Unknown | ||
| 98 | 21.08 | C35H52O6 | 567.3711[M-H]−(4.4) | 351.2523 | Unknown | |
| 99* | 21.72 | C30H46O3 | 453.3361[M-H] −(−1.8) 499.3418[M-H-HCOOH] −(−1.0) 907.6812[2M-H] −(−0.4) |
435.3265[M-H2O]− | Dehydrotrametenolic acid | Zhu et al., 2018b |
| 100 | 22.29 | C48H42O4 | 681.3003[M-H] −(−0.3) | Unknown | Zhu et al., 2018b | |
| 101 | 22.46 | C35H54O6 | 569.3862[M-H]−(3.5) 615.3923[M-H+HCOOH]−(4.2) |
16-O-Acetylpachymic acid/ 3β,15α-Bis (acetyloxy)-24-dien-21-oic acid |
Zou et al., 2019
Wang, 2016; |
|
| 102 | 22.69 | C31H48O3 | 467.3516[M-H] −(−1.9) 513.3594,[M-H+HCOOH]−(2.7) |
371.2554, 339.2668 |
Dehydroeburicoic acid isomer | |
| 103 | 22.98 | C30H48O3 | 455.3515[M-H] −(−2.2) 501.3605[M-H+HCOOH]−(5.0) |
437.3315 | Trametenolic acid/Oleanolic acid | Wang, 2016 |
| 104 | 23.37 | C30H48O3 | 455.3515[M-H] −(−2.2) 501.3605[M-H+HCOOH]−(5.0) |
437.3315 | Trametenolic acid/Oleanolic acid | Zhu et al., 2018b |
| 105 | 24.03 | C21H34O3 | 333.2399[M-H] −(−9.3) | Unknown | ||
| 106* | 24.43 | C31H48O3 | 467.3516[M-H] −(−1.9) 513.3594[M-H-HCOOH]−(2.7) 935.7140[2M-H]−(1.2) |
371.2554, 337.2531, 339.2668 279.2256 |
Dehydroeburicoic acid | Zhu et al., 2018b |
| 107 | 24.72 | C30H44O3 | 451.3209[M-H] −(−0.7) 497.3270[M-H+HCOOH]−(0.6) | 409.3091 325.1786 |
Porilactone A/Porilactone B | Chen et al., 2019 |
| 108 | 25.09 | C22H40O4 | 367.2874[M-H]−(7.1) | Unknown | ||
| 109* | 25.78 | C31H50O3 | 469.3668[M-H] −(−3.0) 515.3738[M-H]−(0.4) |
453.3492 373.2771 339.2668 279.2227 |
Eburicoic acid | Zhu et al., 2018b; Wang, 2016 |
| 110 | 26.20 | C40H60O5 | 619.4365[M-H]−(0.5) 665.4432[M-H+HCOOH]−(2.3) |
469.3668 465.3363 |
Unknown | |
| 111 | 26.60 | C18H34O2 | 281.2469[M-H] −(−4.3) | Unknown | ||
| 112 | 27.22 | C31H46O3 | 465.3363[M-H] −(−1.3) 511.3397[M-H+HCOOH] −(−5.1) |
Unknown | Chen et al., 2019 | |
| 113 | 27.62 | C31H46O3 | 465.3363[M-H] −(−1.3) 511.3397[M-H+HCOOH] −(−5.1) |
Unknown | Wang, 2016 | |
| 114 | 28.09 | C31H48O3 | 467.3516[M-H] −(−1.9) | Dehydroeburicoic acid isomer | ||
| 115 | 28.31 | C27H28O8 | 479.1717[M-H]−(2.7) | Unknown | ||
| 116 | 28.87 | C24H44O7 | 443.3006[M-H] −(−0.7) | Unknown | ||
| 117 | 29.23 | C33H62O10 | 617.4265[M-H] −(−2.9) | Unknown | ||
| 118 | 29.43 | C30H50O4 | 473.3612[M-H] −(−4.0) | Unknown | ||
| 119 | 29.56 | C32H48O4 | 495.3483[M-H]−(1.8) 541.3482[M-H+HCOOH] −(−8.7) |
473.3612 | Poricoic acid CM | Zhu et al., 2018b |
*
Identified by comparing with standard compounds.