FIGURE 5.

Unmasked BPA and BPAF effects on RACK1 promoter. THP-1 cells were transiently transfected with the Δ1 construct (A, C) or the pGL4.32 [luc2P/NF-κB-RE/Hygro] vector reporter construct (B). (A, C) After transfection, cells were pre-treated for 30 min with 20 μM mifepristone (Mife) with or without 50 μM flutamide (Flut) (A, C) and 1 h with 10 μM Bay 11–7085 (Bay) (A), then treated with 10 μM BPA (A) or 10 μM BPAF (C) for 16 h. Cells were lysed, and luciferase activity was measured as described in “Materials and Methods” section. Luciferase activities are expressed as RLU% respected to non-treated construct (considered as 100%). Results are expressed as mean ± SEM, n = 3 independent experiments performed in triplicate. Statistical analysis was performed with Tukey’s multiple comparison test with **p < 0.01 vs Mife 20 μM + Flu 50 μM, ^§§ p < 0.01 vs Mife 20 μM + Flu 50 μM and ^## p < 0.01 vs Mife 20 μM + Bay 10 μM. B After transfection, cells were treated for 16 h with 10 μM BPA or DMSO as vehicle control (CTRL). Results are expressed as mean ± SEM, n = 3 independent experiments performed in triplicate. Significance was set at p < 0.05 by the Student’s t-test (*p < 0.05).