FIGURE 8.

Role of AR in BPS-induced RACK1 regulation. (A) THP-1 cells transiently transfected with the Δ1 construct were pre-treated for 1 h with 50 μM flutamide, then with 10 μM BPS for 16 h or DMSO as vehicle control (CTRL). Cells were lysed and luciferase activity was measured as described in “Materials and Methods” section. Luciferase activities are expressed as RLU% respected to non-treated construct (considered as 100%). Results are expressed as mean ± SEM, n = 3 independent experiments in triplicate. Statistical analysis was performed with Dunnett’s multiple comparison test with ***p < 0.001 vs control (CTRL). (B, C) THP-1 cells pre-treated for 1 h with 50 μM flutamide for 18 h (B) or 24 h (C) with 10 μM BPS or DMSO as vehicle control (CTRL). (B) mRNA levels evaluated by qPCR (endogenous reference, 18S). (C) The image is a representative Western blot. RACK1 protein levels evaluated by Western blotting, normalized to β-tubulin expression. Each value represents the mean ± SEM, n = 3 independent experiments. Statistical analysis was performed with Dunnett’s multiple comparison test with **p < 0.01; ***p < 0.001 vs control (CTRL).