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. 2021 Sep 30;65(3):3224. doi: 10.4081/ejh.2021.3224

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Meg3 regulated GPX4 via p53. A,B) RT-qPCR analysis showed that the overexpression and knockdown of p53 had no impact on FTH1 and ACSL4 expressions. C) The impact of p53 overexpression and knockdown on GPX4 expression was evaluated using RTqPCR analysis. D,E) Western blot images and quantitative analysis showed the effect of p53 overexpression and knockdown on GPX4 protein expression. F,G) Western blot images and quantitative analysis showed that the overexpression of p53 also reversed the impact of si-Meg3 on the protein expression of GPX4. H,I) Chip assay suggested that p53 significantly bound to GPX4 promoter in injury elicited by OGD + HG; anti-histone-3 (α-H3) served as a positive control, and IgG was used as a negative control. Data were expressed as the mean ± SD. The experiments were carried out for three times. *p<0.05 vs the OGD-HG+ Meg3-NC group; $p<0.05 vs the OGDHG+ Meg3-siRNA group; #p<0.05 vs the Meg3-NC group.