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. 2021 Aug 17;22(10):e52457. doi: 10.15252/embr.202152457

Figure 4. MPK3/6‐mediated ARR1/10/12 phosphorylation in vivo .

Figure 4

  • A–C
    Immunoblot analysis with anti‐MYC antibody or anti‐GFP antibody showing the upshift of ARR1, ARR10, and ARR12 in a phos‐tag gel. The phosphorylated ARR1, ARR10, and ARR12 were abolished after treatment with calf intestinal alkaline phosphatase (CIAP). Seven‐day‐old seedlings of 35S:ARR1:MYC (A), 35S:ARR10:YFP (B), and 35S:ARR12:YFP (C) in Col and MPK3SR backgrounds were treated with or without 200 mM NaCl for indicated time points. Total proteins were extracted and subjected to immunoblot analysis. ARR1, ARR10, and ARR12 proteins separated on SDS–PAGE gel without phos‐tag were detected as loading controls.

Data information: Similar results were obtained with three biological repeats.