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. 2021 Aug 17;22(10):e52457. doi: 10.15252/embr.202152457

Figure 5. Salt stress‐induced ARR1/10/12 degradation is dependent on MPK3/6.

Figure 5

  • A–C
    Immunoblot analysis the effects of MPK3/6 on salt stress‐induced ARR1/10/12 protein degradation. Seven‐day‐old seedlings of 35S:ARR1:MYC, 35S:ARR10:YFP, and 35S:ARR12:YFP in Col‐0 and MPK3SR backgrounds were treated with or without 200 mM NaCl for indicated time points. Actin was used as the internal reference. The relative intensity of band detected by anti‐MYC or anti‐GFP antibody to that by anti‐Actin antibody without treatment was set to 1.0.
  • D–I
    Quantification analysis of MPK3/6 effect on salt stress‐induced ARR1/10/12 protein degradation in (A–C).

Data information: In (D–I), the statistical analyses of the related density of Western blotting bands were performed using ImageJ software based on three independent biological replicates. Values are means ± SD (n = 3). **, ***, and **** indicate significant difference to the corresponding controls with P < 0.01, P < 0.001, and P < 0.0001, respectively (Student's t‐test).