Figure 6. MPK3/6 promote ARR1/10/12 protein degradation under salt stress.

• A–C
  Immunoblot analysis stability of ARR1/10/12 and ARR1^T553A/10^S383A/12^S323A proteins under salt stress. Seven‐day‐old seedlings of 35S:ARR1:MYC, 35S:ARR10:YFP, 35S:ARR12:YFP, 35S:ARR1^T553A:MYC, 35S:ARR10^S383A:YFP, and 35S:ARR12^S323A:YFP were treated with or without 200 mM NaCl for indicated time points. Actin was used as the internal reference. The relative intensity of band detected by anti‐MYC or anti‐GFP antibody to that by anti‐Actin antibody without treatment was set to 1.0.
• D–I
  Quantification analysis of stability of ARR1/10/12 and ARR1^T553A/10^S383A/12^S323A proteins under salt stress in (A–C).

Data information: In (D–I), the statistical analyses of the related density of Western blotting bands were performed using ImageJ software based on three independent biological replicates. Values are means ± SD (n = 3). **, ***, and **** indicate significant difference to the corresponding controls with P < 0.01, P < 0.001, and P < 0.0001, respectively (Student's t‐test).

Source data are available online for this figure.