Figure 10. MPK3/6 act upstream of ARR1/10/12 in response to salt stress.

• A–D
  Three‐day‐old seedlings of Col‐0, MPK3SR, MPK3SRarr1/12, and arr1/12 were transferred to 0.5 μM NA‐PP1‐containing 1/2 MS medium for 2 days to inhibit MPK3^TG in MPK3SR and MPK3SRarr1/12, and then, the plants were transferred to 0.2 μM NA‐PP1‐containing 1/2 MS medium supplemented with or without 50 mM or 75 mM NaCl. Pictures were taken, and the elongated root length was determined 3 days later (A–B). Changes in survival rates (C) and fresh weights (D) were examined 10 days later. The fresh weight of NaCl treated seedlings/fresh weight of NaCl untreated seedlings ratios were labeled in the columns in (D). Scale bar, 1 cm.

Data information: In (B–D), data are means of three biological replicates ± SD (n = 60 for (B), and n = 12 for (C) and (D)). **, ***, and **** indicate significant difference to the corresponding controls with P < 0.01, P < 0.001, and P < 0.0001, respectively (Student's t‐test).