Figure 1. Compartment‐specific localization of neuronal transcripts and proteins.

1. Schematic of the workflow for the culture of primary hippocampal neurons and 48 h of PTX treatment.
2. Quantification of miniature EPSC amplitudes in mock‐ and PTX‐treated hippocampal neurons. (n = 10 neurons per condition from three independent experiments, independent two‐sample Wilcoxon–Mann–Whitney U‐test, *P < 0.05).
3. Volcano plots demonstrating enrichment of transcripts in either the somatic (red) or process (yellow) compartment with representative genes highlighted.
4. Top Gene Ontology Pathway Analysis for transcripts enriched in the somatic and process compartment.
5. Volcano plots demonstrating enrichment of proteins in either the somatic (red) or process (yellow) compartment with representative genes highlighted.
6. Quantification of the highlighted genes enriched in either the somata or processes at the transcript or protein level. (Upper row: Scatterplots of transcript level; n = 2; crossbar represents mean. Lower row: Boxplot of protein levels; n = 4). Boxplots: central line: median; box: 25^th to 75^th percentile; whiskers: until last data point within 1.5× interquartile range (IQR).

Linear model was used to fit transcriptome and proteome data and subsequently contrasted using likelihood ratio testing (transcriptome) or empirical Bayes statistics (proteome) for compartment effects.