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. 2021 Aug 2;22(10):e52301. doi: 10.15252/embr.202052301

Figure 3. Suprabasal BSCs are prone to detachment‐induced apoptosis.

Figure 3

  1. GO classification of differentially regulated genes in P50 suprabasal BSCs (CD34+/Itgα6low) compared with basal BSCs (CD34+/Itgα6high) clustered for biological process (clustering and statistical analysis done with Metascape, according to Zhou et al, 2019). Columns of interest in red.
  2. Functional classification of selected differentially expressed genes in sorted suprabasal BSC vs basal BSC after RNAseq analysis (red: upregulated genes, green: downregulated genes; fold change > 2, P < 0.05; statistical analysis done according to Wagle et al, 2015).
  3. qRT–PCR for mRNA expression of the anoikis‐related markers Bim and Bmf in sorted n‐B (non‐bulge), bBSC (basal BSCs) and sbBSC (suprabasal BSCs; n = 3 biological replicates; mean ± standard error of mean [SEM]; *P < 0.05, ns, not significant, two‐way ANOVA test).