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. 2021 Aug 2;22(10):e52301. doi: 10.15252/embr.202052301

Figure 5. Bcl‐2 blocks apoptosis during catagen of the hair follicle cycle.

Figure 5

  • A
    Histology of P19 catagen back skin HF of control (Ctr) and Bcl‐2EOE mice. RS: retracting strand, Scale bar, 50 µm.
  • B
    Analysis of length and width of retracting strands of control (Ctr) and Bcl‐2EOE mice (n = 4; multiple measurements/biological replicate; whiskers: min and max; boxes: median ± first and third quartile; ****P < 0.0001, two‐tailed unpaired Student's t‐test).
  • C
    Itgα6 and aCas3 immunofluorescence staining of catagen tail skin HF of control (Ctr) and Bcl‐2EOE mice (n = 4 biological replicates). Dashed lines: retracting strands. Scale bar, 100 µm.
  • D
    Quantification of aCas3+ cells in the RS of P19 Ctr and Bcl‐2EOE mice (n = 4 biological replicates; mean ± standard deviation [SD]; ****P < 0.0001, two‐tailed unpaired Student's t‐test). HF, hair follicle.
  • E
    Hair‐coat recovery in Ctr and Bcl‐2EOE mice following depilation (n = 3 biological replicates).
  • F, G
    qRT–PCR for mRNA expression of the quiescence‐related markers Bmp6 (F) and Fgf18 (G) in epidermis of control (Ctr) and Bcl‐2EOE mice (P27), (n = 4 biological replicates; mean ± standard error of mean [SEM]; *P < 0.05, ***P < 0.001, two‐tailed unpaired Student's t‐test).