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Mutation types at each target site of multiplex edited T1 plants with GBVS. A, The structure of the pEC1-GL2 plasmid with multiple sgRNAs. The expression of sgRNAs for GL2 and the target loci (PYR1, PYL1, and PYL2) was driven by the U6-26, U6-29, and U6-1 promoters, respectively. HygR, hygromycin resistance gene; RB and LB, T-DNA right and left borders, respectively. B, Mutation types of PYR1, PYL1, and PYL2 loci were analyzed in individual T1 plants showing glabrous phenotype.
