Table 1.
Segregation patterns of homozygous and biallelic T1 mutations
| Line | T1 |
T2 |
||||||
|---|---|---|---|---|---|---|---|---|
| Mutation Type | Indels | Number of Lines Tested | M1 (Homozygote) | M2 （Homozygote） | M1 and M2 (Biallele) | Number of New Mutations | Number of Reversions | |
| jaz1 #4 | Biallele | M1: D (TTGACA) | 20 | 5 | 6 | 9 | 0 | 0 |
| M2: I (A) | ||||||||
| jaz1 #19 | Homozygote | M1: D (ACAGAACTTCCTATTG) | 20 | 20 | 0 | 0 | 0 | 0 |
| jaz1 #33 | Homozygote | M1: D (CATTGAC) | 20 | 20 | 0 | 0 | 0 | 0 |
| jaz1 #41 | Homozygote | M1: D (ACAGAACTTCCTATTG) | 20 | 20 | 0 | 0 | 0 | 0 |
| ap1 #10 | Homozygote | M1: D (T) | 20 | 20 | 0 | 0 | 0 | 0 |
| ap1 #33 | Biallele | M1: I (T) | 20 | 6 | 5 | 9 | 0 | 0 |
| M2: I (C) | ||||||||
| ap1 #59 | Homozygote | M1: D (T) | 20 | 20 | 0 | 0 | 0 | 0 |
| gai #3 | Homozygote | M1: D (GTT) | 20 | 20 | 0 | 0 | 0 | 0 |
| gai #27 | Biallele | M1: D (T) | 20 | 5 | 7 | 8 | 0 | 0 |
| M2: D (AGCTGTTCT) | ||||||||
| gai #56 | Biallele | M1: D (G) | 20 | 4 | 9 | 7 | 0 | 0 |
| M2: D (TT) | ||||||||
| tt4 #8 | Biallele | M1: I (A) | 20 | 6 | 6 | 8 | 0 | 0 |
| M1: D (CAA) | ||||||||
Twenty T2 seedlings from each T1 homozygous or biallelic line were genotyped by sequencing. M1 and M2 indicate the type of mutation in T1 plants. D indicates deletion and I indicates insertion at target sites.