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. 2021 May 24;187(2):632–645. doi: 10.1093/plphys/kiab240

Figure 1.

Figure 1

Fusion of CBCR “GAF” domains with DGC “GGDEF” domain of Tlr0924 (SesA). A, Domain architecture of Tlr0924Δ. B, Alignment of fusion protein constructs, color-coded by coding sequence (Tlr0924, pale green; RcaE, pink; NpR6012g4, cyan; dark orange box, short repeated motif). The alignment starts with the last β strand of the GAF domain (LWGLLIAH in Tlr0924Δ), with the GAF domain ending in the vicinity of VALQQ in the same sequence and with the GGDEF domain in the vicinity of NLQ in the same sequence. Numbering is based on full-length Tlr0924 (GenBank accession BAC08476). C, DGC activity of fusion protein constructs (indicated as in panel B) in 15Z (blue) and 15E (orange) photostates. Error bars are drawn at one standard deviation (n = 3). No construct exhibited significant light-regulated DGC activity (P < 0.05).