Table 2.
Metabolic activities of HIS1 and various HSL proteins for TE, BBC-OH, and TFT
| Protein | Activity (U/µg) |
||
|---|---|---|---|
| TE | BBC-OH | TFT | |
| HIS1 | 174.14 ± 1.25 | 50.45 ± 1.28a | 159.16 ± 3.29a |
| OsHSL1 | <0.1 | 4.57 ± 0.49a | 133.01 ± 8.3a |
| OsHSL2 | 72.84 ± 0.73 | <0.1 | <0.1 |
| OsHSL4 | 17.55 ± 0.30 | <0.1 | <0.1 |
| OsHSL5 | <0.1 | <0.1 | <0.1 |
| OsHSL6 | <0.1 | <0.1 | <0.1 |
| TaHSL1 | 0.92 ± 0.14 | <0.1 | <0.1 |
| TaHSL2 | 3.81 ± 0.70 | <0.1 | <0.1 |
The specific activity of recombinant HIS1 and HSL proteins for each compound was determined from the estimated amount of protein in the translation reaction mixture as previously described (Maeda et al., 2019). One unit (U) of enzyme activity is defined as the conversion of 1 μmol of substrate per minute at 30°C under the assay conditions specified in “Materials and methods.” Specific activity was calculated as units per microgram of protein. Data are means ± sd for three separate reactions performed with the same preparation of protein. aData are from a previous study (Maeda et al., 2019).