Table 3.
BRCA2 Site-Directed Mutagenesis Primers
| Nucleotide change | Amino acid change | Forward mutagenesis primer | Reverse mutagenesis primer |
|---|---|---|---|
| c.3055C>G | p.(L1019V) | 5′-CAGCTTCAAATAAGGAAATCAAGGTCTCTGAACATAACATTAAGAAG-3′ | 5′-CTTCTTAATGTTATGTTCAGAGACCTTGATTTCCTTATTTGAAGCTG-3′ |
| c.4570T>G | p.(F1524V) | 5′-AACCTACTCTGTTGGGTGTTCATACAGCTAGCGGG-3′ | 5′-CCCGCTAGCTGTATGAACACCCAACAGAGTAGGTT-3′ |
| c.6220C>A | p.(H2074N) | 5′-AGCAAGTTTCCATTTTAGAAAGTTCCTTAAACAAAGTTAAGGGAGTG-3′ | 5′-CACTCCCTTAACTTTGTTTAAGGAACTTTCTAAAATGGAAACTTGCT-3′ |
| c.7415A>C | p.(K2472T) | 5′-TCAAGCAGCAGCTGTAACTTTCACAACGTGTGAAGAAGAAC-3′ | 5′-GTTCTTCTTCACACGTTGTGAAAGTTACAGCTGCTGCTTGA-3′ |
| c.7544C>T | p.(T2515I) | 5′-GGCAGTCTGTATCTTGCAAAAATATCCACTCTGCC-3′ | 5′-GGCAGAGTGGATATTTTTGCAAGATACAGACTGCC-3′ |
| c.7879A>T | p.(I2627F) | 5′-GGTTTATAATCACTATAGATGGTTCATATGGAAACTGGCAGCTAT-3′ | 5′-ATAGCTGCCAGTTTCCATATGAACCATCTATAGTGATTATAAACC-3′ |
| c.7940T>C | p.(L2647P) | 5′-ATTTGCTAATAGATGCCCAAGCCCAGAAAGGGTGC-3′ | 5′-GCACCCTTTCTGGGCTTGGGCATCTATTAGCAAAT-3′ |
| c.7958T>C | p.(L2653P) | 5′-CTAAGCCCAGAAAGGGTGCCTCTTCAACTAAAATACAGAT-3′ | 5′-ATCTGTATTTTAGTTGAAGAGGCACCCTTTCTGGGCTTAG-3′ |
| c.8063T>C | p.(L2688P) | 5′-CAGCTGCAAAAACACTTGTTCCCTGTGTTTCTGACATAATTTC-3′ | 5′-GAAATTATGTCAGAAACACAGGGAACAAGTGTTTTTGCAGCTG-3′ |
| c.8165C>G | p.(T2722R) | 5′-CCCAAAAAGTGGCCATTATTGAACTTAGAGATGGGTGGTATG-3′ | 5′-CATACCACCCATCTCTAAGTTCAATAATGGCCACTTTTTGGG-3′ |
| c.8167G>C | p.(D2723H) | 5′-AGTGGCCATTATTGAACTTACACATGGGTGGTATGCTG-3′ | 5′-CAGCATACCACCCATGTGTAAGTTCAATAATGGCCACT-3′ |
| c.8187G>T | p.(K2729N) | 5′-AGATGGGTGGTATGCTGTTAATGCCCAGTTAGATC-3′ | 5′-GATCTAACTGGGCATTAACAGCATACCACCCATCT-3′ |
| c.8243G>A | p.(G2748D) | 5′-GAATGGCAGACTGACAGTTGATCAGAAGATTATTCTTCATG-3′ | 5′-CATGAAGAATAATCTTCTGATCAACTGTCAGTCTGCCATTC-3′ |
| c.8525G>A | p.(R2842H) | 5′-ATCATCTGGATTATACATATTTCACAATGAAAGAGAGGAAGAAAAGG-3′ | 5′-CCTTTTCTTCCTCTCTTTCATTGTGAAATATGTATAATCCAGATGAT-3′ |
| c.8567A>C | p.(E2856A) | 5′-AGCAGCAAAATATGTGGCGGCCCAACAAAAGAGAC-3′ | 5′-GTCTCTTTTGTTGGGCCGCCACATATTTTGCTGCT-3′ |
| c.9371A>T | p.(N3124I) | 5′-CATATGTTAATTGCTGCAAGCATCCTCCAGTGGCG-3′ | 5′-CGCCACTGGAGGATGCTTGCAGCAATTAACATATG-3′ |
Complete list of all BRCA2 mutagenesis primers used in this study. Human Genome Variant Nomenclature (HGVS) nucleotide and protein numbering shown for BRCA2 reference sequence (https://www.ncbi.nlm.nih.gov/genbank; GenBank accession number NM_000059.3).