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. Author manuscript; available in PMC: 2021 Oct 5.
Published in final edited form as: FASEB J. 2021 Mar;35(3):e21415. doi: 10.1096/fj.202002540R

FIGURE 2.

FIGURE 2

SphK1 deletion protects from GalN/LPS-induced apoptosis. A-C, WT, SphK1−/−, and SphK2−/− mice were treated without or with GalN/LPS. (A,B) After 4 hours, liver sections were TUNEL stained to visualize apoptosis and quantified as described in Materials and Methods. Data are expressed as arbitrary units (AU) and are means ± SEM. n = 3. * P < .01 compared to WT. C, Proteins in liver lysates were separated by SDS-PAGE and analyzed by immunoblotting with antibody for cleaved caspase 3. Blots were re-probed with anti-GAPDH to show equal loading and transfer