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Effect of inflammatory stimuli on endogenous and exogenous PRG4 immunolocalization. Immunolocalization of PRG4 in hTCEpi cells with anti-PRG4 Ab LPN (A), and those treated with 10 ng/ml IL-1β (B) or 100 ng/ml TNFα (C). Confocal microscopy visualization of hTCEpi cells treated with FITC-tagged PRG4 (D), along with IL-1β (E), or TNFα (F). Nuclei are stained with DAPI. Scale bar is 10 μm, 63X magnification for A-C, 63X (x2) magnification for D-F.
