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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Exp Eye Res. 2021 May 25;208:108628. doi: 10.1016/j.exer.2021.108628

Figure 4.

Figure 4.

Effect of rhPRG4 on in vitro exogenous MMP-9 activity and endogenous gene expression, as well as binding between MMP-9 and rhPRG4. rhPRG4 incubated with or without MMP-9 for 60 minutes at 37C (A). Exogenous MMP-9 activity with 150 μg/mL, 300 μg/mL, and 450 μg/mL of rhPRG4 in solution (B) or in the presence of normal human tears (C). Surface plasmon resonance sensograms of binding between rhMMP-9 and rhPRG4 (D). Endogenous MMP-9 expression in hTCEpi cells with or without inflammatory treatment and rhPRG4. Samples normalized to housekeeping gene 18s and to control samples using ΔΔCt method (E). * p < 0.05, *** p < 0.001. Data are mean ± SEM (n=4) for (A-C) and n=3 for (E).