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Effect of experimental dry eye (EDE) on PRG4 expression in the cornea and the lacrimal gland in vivo. PRG4 in mouse cornea immunolocalized with anti-PRG4 Ab LPN from untreated mice (A) and mice with EDE (B), quantified using ImageJ, * p < 0.05, n = 4 (C). Lacrimal gland lysate analyzed by western blot using anti-PRG4 Ab LPN as primary antibody (D), densitometry of relative fold change in PRG4 (E). Data are mean ± SEM (n = 3–5).
