Table 1.

Effect of rhPRG4 on proinflammatory cytokine and chemokine secretion with inflammatory stimuli. The data is presented as fold change from control, with inflammatory stimuli (100 ng/mL TNFα, or 10 ng/mL IL-1β) with or without 300 μg/mL of rhPRG4. For comparing TNFα or IL-1β to CTRL (no rhPRG4)

Cytokine / Chemokine	Control (pg/ml)	Fold Change in Cytokine/Chemokine Production over Control
		Control + rhPRG4	IL-1β	IL-1β + rhPRG4	TNFα	TNFα + rhPRG4
IP10	329 ± 74.3	0.797 ± 0.237	1.68 ± 0.29	0.980 ± 0.267	3.72 ± 0.775##	1.96 ± 0.810**
RANTES	28.2 ± 3.05	0.744 ± 0.314	2.50 ± 0.67	1.201 ± 0.466	3.90 ± 1.18###	1.65 ± 0.566*
ENA-78	44.8 ± 18.1	0.368 ± 0.169	10.8 ± 4.43#	4.611 ± 2.353*	5.99 ± 1.31###	1.18 ± 0.303***
GROα	339 ± 80.9	0.559 ± 0.117*	1.60 ± 0.137###	0.836 ± 0.098***	3.25 ± 0.136###	1.37 ± 0.24***
IL-6	1320 ± 327	0.705 ± 0.078**	4.92 ± 1.92	5.52 ± 2.37	15.0 ± 6.56#	13.3 ± 6.11
IL-8	(2.33 ± 1.14) x 104	0.731 ± 0.082[*]	7.11 ± 3.00	5.96 ± 2.69	9.69 ± 3.70##	7.90 ± 3.62
MIP-3α	57.8 ± 9.05	0.388 ± 0.092**	1.16 ± 0.09	0.442 ± 0.088***	3.21 ± 0.27###	1.56 ± 0.270***
MIG	5.42 ± 1.17	0.707 ± 0.131	4.45 ± 1.62#	4.42 ± 1.82	6.00 ± 2.15##	0.974 ± 0.149*
MIP-1α	56.3 ± 25.5	0.540 ± 0.206[*]	49.4 ± 21.7[#]	56.3 ± 25.3	123.6 ± 57.7##	9.05 ± 5.35[*]
MIP-1β	9.13 ± 4.71	0.632 ± 0.211	68.3 ± 30.5#	68.3 ± 30.5	109.1 ± 49.3##	10.6 ± 4.23[*]

^#p < 0.05

^##p < 0.01

^###p < 0.001, and

^[#]0.05 < p < 0.08. For comparing with rhPRG4 to without rhPRG4 within each group (CTRL, IL-1β or TNFα)

^*p < 0.05

^**p < 0.01

^***p < 0.001, and

^[*]0.05 < p < 0.08. Data are mean ± SEM (n=6–10).