TABLE 2.
Transactivation and repression properties of GAL4-Pax4 chimeras in various cell typesa
| Constructa | Relative activity (mean ± SD)
|
||
|---|---|---|---|
| GAL4 DBD | GAL4–Pax4 232–349b | GAL4–Pax4 232–314b | |
| (GAL4)5E1bTATALucc | |||
| 293 | 1.11 ± 0.3 | 15.7 ± 1.7 | 35.1 ± 9.9 |
| 293T | 1.44 ± 0.2 | 12.7 ± 1.9 | 42.4 ± 9.4 |
| F9 | 1.07 ± 0.3 | 0.37 ± 0.15 | 11.7 ± 5.4 |
| (GAL4)4TKLucc | |||
| βTC1 | 102 ± 10.5 | 30.9 ± 10.3 | 97.2 ± 16.6 |
| αTC1 | 101 ± 14.2 | 61.0 ± 29.1 | 98.4 ± 15.2 |
| HIT-T15 | 106 ± 23.4 | 49.7 ± 5.7 | 144 ± 34.0 |
| HeLa | 98 ± 9.0 | 15.5 ± 3.5 | 73.7 ± 24.9 |
| CHO-K1 | 101 ± 6.9 | 7.2 ± 0.9 | 85.0 ± 6.2 |
a
Two micrograms of an expression plasmid (pcDNA, GAL4 DBD, GAL4–Pax4 232–349, or GAL4–Pax4 232–314), 1 μg of pEFBOSβ-Gal, and 2 μg of a GAL4 reporter plasmid [(GAL4)5E1bTATALuc or (GAL4)4TKLuc] were used. The data are presented as activity relative to that obtained in cells transfected with the empty vector (pcDNA3), arbitrarily set at 1 [for (GAL4)5E1bTATALuc] or 100 [for (GAL4)4TKLuc].
b
Structure is illustrated in Fig. 8.
c
Structure and origin are described in Materials and Methods.