TABLE 3.

Effects of adenoviral E1A products on the transactivation potential of Pax4^a

Plasmid	Relative activity (mean ± SD [n = 3])
	pRSVH2O	pRSV5E1Ac	pRSVJF12d
GAL4 DBD	104 ± 7.8	111 ± 13	106 ± 6.6
GAL4–Pax4 232–349b	7.1 ± 1.2	60 ± 20.8	17 ± 7.4
GAL4–Pax4 232–314b	138 ± 28.0	495 ± 35.0	160 ± 18.5
GAL4–Pax4 278–349b	10 ± 2.1	18 ± 4.2	18 ± 3.3

^aOne microgram of an E1A expression plasmid (pRSVH₂O [empty vector], pRSV5E1A, or pRSVJF12), 1 μg of a GAL4-Pax4 chimera expression plasmid (pcDNA, GAL4 DBD, GAL4–Pax4 232–349, GAL4–Pax4 232–314, or GAL4–Pax4 278–349), 2 μg of (GAL4)₄TKLuc, and 1 μg of pEFBOSβ-Gal were transiently transfected into CHO-K1 cells. The data are presented as activity relative with that obtained in cells transfected with the empty vector (pcDNA3), arbitrarily set at 100. 

^bStructure is illustrated in Fig. 8. 

^cExpression plasmid for wild-type E1A including both 12S- and 13S-type E1A. 

^dExpression plasmid for 12S-type E1A.