FIGURE 4.

In vitro cytolytic activities of 5T4‐targeted CAR T cells. The cytotoxicity of T cells expressing 5T4‐specific CAR was determined by coculture with three 5T4⁺ ovarian cancer cell lines and one 5T4⁻ cell line at varying E/T ratios (1:1, 5:1, and 10:1) for 24 h, respectively. (A) Cell lysis was determined by a standard nonradioactive cytotoxic assay. 5T4 CAR‐T cells can specifically kill 5T4 positive tumor cells but not the negative cells. Each data reflect the mean ± SD of triplicates. (B) The cytokine concentration in the media was determined by ELISA. 5T4 CAR‐T cells produced IL‐2, IFN‐γ, and GM‐CSF after stimulation with 5T4‐positive tumor cells. No cytokine release was seen with NT T cells