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. 2021 Sep 21;12:732344. doi: 10.3389/fpls.2021.732344

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Copyright © 2021 Holmes, Lung, Collyer and Punja.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Root development on shoots after 8–10 weeks of growth in tissue culture and acclimatization to produce plants. (A,B) Spontaneous development of roots on nodal explants. (C) Plantlets were removed from tissue culture and transferred to coco growth media and placed under high humidity conditions for 2 weeks. (D,E) Acclimatization of plantlets from meristems of genotype MBD in different growth substrates. Plantlets were removed from tissue culture jars after 12 weeks of growth on the MM and placed into rockwool or peat under humid conditions for 14 days. Rockwool or peat plugs were soaked in a fertilizer mix of 1 ml/L of pH Perfect® Sensi Grow A&B and CALiMAGIc (General Hydroponics, Santa Rosa, CA, United States) in ~pH 5.8 water. (F,G) Growth of plants on a coco potting medium under ambient conditions following successful acclimatization. (H) Hydroponic system filled with 8 L of the fertilizer mix with vigorous growth of plants derived from meristem tissue cultures.